FRET-Based Nanobiosensors for Imaging Intracellular Ca2+ and H+ Microdomains
نویسندگان
چکیده
منابع مشابه
FRET-Based Nanobiosensors for Imaging Intracellular Ca2+ and H+ Microdomains
Semiconductor nanocrystals (NCs) or quantum dots (QDs) are luminous point emitters increasingly being used to tag and track biomolecules in biological/biomedical imaging. However, their intracellular use as highlighters of single-molecule localization and nanobiosensors reporting ion microdomains changes has remained a major challenge. Here, we report the design, generation and validation of FR...
متن کاملIntracellular FRET-based probes: a review.
Probes that exploit Förster resonance energy transfer (FRET) in their feedback mechanism are touted for their sensitivity, robustness, and low background, and thanks to the exceptional distance dependence of the energy transfer process, they provide a means of probing lengthscales well below the resolution of light. These attributes make FRET-based probes superbly suited to an intracellular env...
متن کاملCa2+ Microdomains Organized by Junctophilins
Excitable cells typically possess junctional membrane complexes (JMCs) constructed by the plasma membrane and the endo/sarcoplasmic reticulum (ER/SR) for channel crosstalk. These JMCs are termed triads in skeletal muscle, dyads in cardiac muscle, peripheral couplings in smooth and developing striated muscles, and subsurface cisterns in neurons. Junctophilin subtypes contribute to the formation ...
متن کاملCa2+ Microdomains in T-Lymphocytes
Early Ca2+ signaling is characterized by occurrence of Ca2+ microdomains formed by opening of single or clusters of Ca2+ channels, thereby initiating first signaling and subsequently activating global Ca2+ signaling mechanisms. However, only few data are available focusing on the first seconds and minutes of Ca2+ microdomain formation and related signaling pathways in activated T-lymphocytes. I...
متن کاملNanobiosensors and fluorescence based biosensors: An overview
A biosensor can sense biological elements after interaction with the recognition element. The signal produced due to interaction of the analyte with its biochemical element is transduced by a transducer and detected by appropriate modes. The miniaturization of these biosensors at the nano level using nanostructures as a platform for sensing the analyte or its detection is called a nanobiosensor...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Sensors
سال: 2015
ISSN: 1424-8220
DOI: 10.3390/s150924662